Structure analysis of group I plant nucleases
نویسندگان
چکیده
منابع مشابه
Structure analysis of group I plant nucleases
Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several d...
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A classification system is presented to distinguish 3 corn nucleases-Ribonuclease I, Ribonuclease II, and Nuclease I-which were described in the first paper of this series. The 2 ribonucleases are specific for RNA, are endonucleases, and liberate purine and pyrimidine cyclic nucleotides from dinucleotide monophosphates as well as from RNA. Ribonuclease I and II hydrolyze the purine cyclic nucle...
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Three enzymes with ribonuclease activity, one of which also had deoxyribonuclease activity, have been isolated and partially purified from corn seeds and seedlings. The purification of Ribonuclease I from mature seed was previously reported. This enzyme has a pH optimum near 5.0, is loosely adsorbed to carboxymethyl-cellulose, and has a molecular weight of 23,000, determined by gel filtration.R...
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Group I introns are mobile, self-splicing genetic elements found principally in organellar genomes and nuclear rRNA genes. The only group I intron known from mitochondrial genomes of vascular plants is located in the cox1 gene of Peperomia, where it is thought to have been recently acquired by lateral transfer from a fungal donor. Southern-blot surveys of 335 diverse genera of land plants now s...
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Gene targeting is a powerful tool for functional gene studies. However, only a handful of reports have been published describing the successful targeting of genome sequences in model and crop plants. Gene targeting can be stimulated by induction of double-strand breaks at specific genomic sites. The expression of zinc finger nucleases (ZFNs) can induce genomic double-strand breaks. Indeed, ZFNs...
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ژورنال
عنوان ژورنال: Journal of Synchrotron Radiation
سال: 2010
ISSN: 0909-0495
DOI: 10.1107/s0909049510030700